3 Background

Background (Conceptual) 25 mins (aim to reduce)

• Very high-level overview of how spatial fits in to existing omics technologies
• Similarities and differences to single cell, bulk, visium
• Basic techs
  • Comparison between techs, which ones to use, why?
• Questions I would like to know as a noob:
  • What’s the most cost-effective tech?
  • Which one should be used for my samples and conditions of interest?
  • Considerations for study design, i.e. how many replicates
• Which tools and packages to use
  • R vs. Python
  • Sfe (biconductor vs seurat)